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Objective To investigate the hydrophilic chemical constituents from Thesium Chinensis Turcz.Methods The water extract of Thesium Chinensis Turcz.was adsorbed onto AB-8 resin column and eluted with 50% ethanol to give the hydrophilic portion TT50.TT50 was further separated and purified by repeated column chromatography on silica gel,Sephadex LH-20.The structures of these purified compounds were identified by NMR spectral analysis and comparison with the reported data.Results Six compounds were isolated and identified as kaempferol(1),kaempferol-3-O-glucoside(2),kaepmferol-3,7-di-O-β-D-glucopyranoside(3),kaempferol-3-O-L-rhamnopyranosyl(1 → 2)-β-D-glucopyranoside(4),kaemperol-3-O-α-L-rham-nopyranosyl(1→2)-[6-O-acetyl]-β-D-glucopyranoside(5),rutinoside(6).Conclusion The main constituents of TT50 were kaempferol glycosides.Compounds 4 and 5 were isolated from this plant for the first time.
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Objective To evaluate the therapeutic effects of Thesium chinensis Turcz extracts on IgA nephropathy (IgAN) rats .Methods The hot water extracted solution of Thesium chinensis Turcz flow through the AB‐8 macroreticular res‐in column repeatedly ,then being eluted with different contents of ethanol to get four part of extract .The IgAN model of SD rats were established by oral intake of bovine serum albumin(BSA ,400 mg/kg) together with injection of lipopolysaccharide(LPS) and CCl4 .Beginning with 8th week ,the positive group(LGT ,10 mg/kg) ,water extract group(TT ,1 .0 g/kg) ,water soluble group(TTW ,0 .4 g/kg) ,high polarity group(TT20 ,0 .4 g/kg) and medium polarity group(TT50 ,0 .4 g/kg) rats were intragas‐tric administrated drug daily ,the normal group control and model control group rats were given with saline(n=8) .During the next 5 weeks treatment process ,the 24 h urine volume ,24 h urine protein concentration ,and quantity of erythrocyte in urine were observed .The levels of TP ,ALP ,ALT ,AST ,Scr and BUN in serum were determined .The expression of IgA deposition was measured by immunofluorescence staining ,and the pathological changes of kidney tissue were assayed after the therapy process .Results After 5 weeks therapy ,the 24 h urine protein concentration ,the quantity of erythrocyte in urine ,and the level of Scr and BUN of TT and TT50 groups were significantly lower than model control .The fluorescence intensities in the me‐sangial area were weaker than model control .Also ,the degrees of renal capsule expansion and mesangial matrix proliferations , and the kidney tubules damage of TT and TT50 groups were weaker than those of model control .Conclusion The water extract and its medium polarity fraction of Thesium chinensis Turcz show good therapeutic effects on IgAN rats .
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Objective:To investigate the water soluble chemical constituent from Tribulus terrestris L..Methods:The raw material was extracted by 80% ethanol and the compound was isolated by means of chromatography on silica gel column.The structure of compound was elucidated by spectral analysis (UV,IR, 1HNMR, 13 CNMR,DEPT,2D-NMR,MS).Results and Conclusion:A water soluble constituent has been isolated from Tribulus terrestris L..Based on the spectroscopic data,it is identified as a new organic acid named terrestric acid (6-amino-4-oxo-1,2,3,4-tetrahydro-1,3,5-triazine-2-carboxylic acid).
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Objective To study the antitumor activities of extract from Ligia exotica(Roux).Methods The dried powder of total Ligia exotica(Roux) was extracted by 37 ℃ water.The solution was concentrated in vacuum,and then was freeze-dried to afford crude extract.The inhibitory effect of the extract on tumor cells proliferation was assayed by MTT method,and transplant tumor model of sarcoma 180(S180) was used.Results The extract from Ligia exotica displayed obvious proliferation inhibitory effect on HeLa,7901,NCI cells,and no growth inhibitory effect on 929 cells in vitro.After administration at the doses of 0.25,0.50,1.00 g?kg-1,ip,for 7d in tumor-bearing mice with S180,The extract caused 26.9 %,45.3 %,64.6 % inhibition rates,respectively.Conclusion The extract from Ligia exotica showed significant antitumor activity.
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Objective To establish a UV method of determination of adenosine in Ligia exotica. Methods Adenosine was extracted with 37℃ water and separated by TLC silica gel, the absorbance was detected at 260nm with UV spectrophotometry.Results The cabibration curve for adenosion was linear within the range of 1.0~20.0?g?mL -1, r=0.9998 (n=6), the average recovery was 99.3%,RSD=1.9% (n=3). Conclusion The method was simple, convenient and accurate for determination of adenosine in Ligia exotica.
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Objective To investigate the alkaloidal constituents of Nauclea officinalis, an anti-inflammatory and antibacterial traditional Chinese herb. Methods The alkaloids were isolated and purified by silica gel, Sephadex LH20, and C-18 ODS column chromatography repeatedly, and their structures were identified by spectral analysis. Results Eleven alkaloids were isolated from the stems of N. officinalis and their structures were identified as angustoline (Ⅰ), 19-O-ethylangustoline (Ⅱ), 3-S-3, 4-dihydroangustoline (Ⅲ), 3-R-3, 4-dihydroangustoline (Ⅳ), naucleamide A (Ⅴ), strictosamide (Ⅵ), vincosamide (Ⅶ), 6′-acetyl-strictosamide (Ⅷ), 2′-acetyl-strictosamide (Ⅸ), pumiloside (Ⅹ), 3-epi-pumiloside (Ⅺ). Conclusion Compounds Ⅰ-Ⅺ are isolated from this plant for the first time.
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Objective:To investigate the constituents of indole alkaloids in Ervatamia yunnanensis and their antiaddictive bioactivities. Methods: The preliminary alkaloids(TEYA) were extracted by 95% EtOH from the stems of Ervatamia yunnanensis.TEYA was then purified by silica gel and Sephadex LH20 column chromatography repeatedly and its structure was identified with spectral analysis.The antiaddictive effects of these indole alkaloids were evaluated by Conditioned Place Preference(CPP) experiments.Results: Nine indole alkaloids were obtained and identified as: coronaridine(1),voacangine(2),3-R-ethoxycoronaridine(3),3-S-ethoxycoronaridine(4), 19-epi-heyneanine(5),heyneanine(6),19-epi-voacristine(7),coronaridine hydroxyindolenine(8) and 12-methoxyl-voaphylline(9).Among them voacangine (2) and 19-epi-voacristine(7) were effective in prevention and treatment of addiction induced by morphine in rats(n=8).Conclusion: Compounds 3,4,8,and 9 have been isolated from Ervatamia yunnanensis for the first time and coronaridine-type indole alkaloids are the main antiaddictive constituents of E.yunnanensis.